0580711 - ÚEM 2024 RIV DE eng J - Journal Article
Goncalves, A.M. - Leal, F. - Moreira, A. - Schellhorn, T. - Hefka Blahnová, Veronika - Zeiringer, S. - Vocetková, Karolína - Tetyczka, C. - Simaite, A. - Buzgo, M. - Roblegg, E. - Costa, P.F.F. - Ertl, P. - Filová, Eva - Kohl, Y.
Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling.
Advanced NanoBiomed Research. Roč. 3, č. 4 (2023). ISSN 2699-9307
R&D Projects: GA MPO(CZ) FV40437
EU Projects: European Commission(XE) 823981 - ActiTOX
Institutional support: RVO:68378041
Keywords : 3R principle * cellulose * electrospinning * epithelial barrier * in vitro modelst * toxicological screening
OECD category: Biomaterials (as related to medical implants, devices, sensors)
Method of publishing: Open access
Result website:
https://onlinelibrary.wiley.com/doi/10.1002/anbr.202200104DOI: https://doi.org/10.1002/anbr.202200104


Herein, intestinal, skin, and pulmonary in vitro tissue models based on electrospun membranes of poly(epsilon-caprolactone) (PCL) and cellulose acetate (CA), cellulose acetate phthalate (CAP), ethylcellulose (EC), or methylcellulose (MC) are presented. Physicochemical characterization and biocompatibility analyses of the scaffolds are carried out using colorectal adenocarcinoma cells (intestine), keratinocytes and fibroblasts (skin), and bronchial and alveolar epithelial cells (lung). PCL, PCL:CA, and PCL:EC are composed of nanofibers, whereas PCL:CAP and PCL:MC scaffolds comprise a combination of micro- and nanofibers. PCL, PCL:CA, PCL:CAP, and PCL:EC samples demonstrate water contact angles greater than 90 degrees and are, therefore, hydrophobic, while PCL:MC mats display a hydrophilic behavior. In intestinal models, cells adhere and proliferate on all scaffolds, in turn, studies with skin cell models reveal that PCL:CA and PCL:CAP blends outperform all other substrates. Lung cell models show that, while 16HBE cells adhere to and proliferate in PCL, PCL:CA, PCL:EC, and PCL:MC scaffolds, A549 cells only have the same biological response on PCL, PCL:CA, and PCL:MC. In summary, all fibrous meshes prepared are biocompatible toward most cell types tested, thus suggesting the potential of PCL-cellulose derivative blends as substrates suitable for in vitro epithelial tissue modeling and toxicity screening.
Permanent Link: https://hdl.handle.net/11104/0351158