Naturally occurring ecdysteroids in triticum aestivum l. And evaluation of fenarimol as a potential inhibitor of their biosynthesis in plants

0546754 - ÚEB 2022 RIV CH eng J - Journal Article
Janeczko, A. - Oklešťková, Jana - Tarkowská, Danuše - Drygaś, B.
Naturally occurring ecdysteroids in triticum aestivum l. And evaluation of fenarimol as a potential inhibitor of their biosynthesis in plants.
International Journal of Molecular Sciences. Roč. 22, č. 6 (2021), s. 1-13, č. článku 2855. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Cold acclimation * Deacclimation * Ecdysteroids * Fenarimol * Plant development * Vernalization * Winter wheat
OECD category: Biochemistry and molecular biology
Impact factor: 6.208, year: 2021
Method of publishing: Open access
http://doi.org/10.3390/ijms22062855

Ecdysteroids (ECs) are steroid hormones originally found in the animal kingdom where they function as insect molting hormones. Interestingly, a relatively high number of these substances can also be formed in plant cells. Moreover, ECs have certain regulatory effects on plant physiology, but their role in plants still requires further study. One of the main aims of the present study was to verify a hypothesis that fenarimol, an inhibitor of the biosynthesis of ECs in the animal kingdom, also affects the content of endogenous ECs in plants using winter wheat Triticum aestivum L. as a model plant. The levels of endogenous ECs in winter wheat, including the estimation of their changes during a course of different temperature treatments, have been determined using a sensi-tive analytical method based on UHPLC-MS/MS. Under our experimental conditions, four substances of EC character were detected in the tissue of interest in amounts ranging from less than 1 to over 200 pg·g−1 FW: 20-hydroxyecdysone, polypodine B, turkesterone, and isovitexirone. Among them, turkesterone was observed to be the most abundant EC and accumulated mainly in the crowns and leaves of wheat. Importantly, the level of ECs was observed to be dependent on the age of the plants, as well as on growth conditions (especially temperature). Fenarimol, an inhibitor of a cytochrome P450 monooxygenase, was shown to significantly decrease the level of naturally occurring ECs in experimental plants, which may indicate its potential use in studies related to the bio-synthesis and physiological function of these substances in plants.
Permanent Link: http://hdl.handle.net/11104/0323145