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Silver ions increase plasma membrane permeability through modulation of intracellular calcium levels in tobacco BY-2 cells

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    0489217 - ÚEB 2019 RIV DE eng J - Journal Article
    Klíma, Petr - Laňková, Martina - Vandenbussche, F. - Van Der Straeten, D. - Petrášek, Jan
    Silver ions increase plasma membrane permeability through modulation of intracellular calcium levels in tobacco BY-2 cells.
    Plant Cell Reports. Roč. 37, č. 5 (2018), s. 809-818. ISSN 0721-7714. E-ISSN 1432-203X
    R&D Projects: GA ČR GA16-10948S
    Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
    Institutional support: RVO:61389030
    Keywords : Auxin * Calcium * Ethylene * Silver ions * Tobacco BY-2 cells * Transmembrane transport
    Subject RIV: ED - Physiology
    OBOR OECD: Cell biology
    Impact factor: 3.499, year: 2018

    Silver ions increase plasma membrane permeability for water and small organic compounds through their stimulatory effect on plasma membrane calcium channels, with subsequent modulation of intracellular calcium levels and ion homeostasis. Abstract: The action of silver ions at the plant plasma membrane is largely connected with the inhibition of ethylene signalling thanks to the ability of silver ion to replace the copper cofactor in the ethylene receptor. A link coupling the action of silver ions and cellular auxin efflux has been suggested earlier by their possible direct interaction with auxin efflux carriers or by influencing plasma membrane permeability. Using tobacco BY-2 cells, we demonstrate here that besides a dramatic increase of efflux of synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA), treatment with AgNO 3 resulted in enhanced efflux of the cytokinin trans-zeatin (tZ) as well as the auxin structural analogues tryptophan (Trp) and benzoic acid (BA). The application of AgNO 3 was accompanied by gradual water loss and plasmolysis. The observed effects were dependent on the availability of extracellular calcium ions (Ca 2+ ) as shown by comparison of transport assays in Ca 2+rich and Ca 2+free buffers and upon treatment with inhibitors of plasma membrane Ca 2+permeable channels Al 3+ and ruthenium red, both abolishing the effect of AgNO 3 . Confocal microscopy of Ca 2+sensitive fluorescence indicator Fluo-4FF, acetoxymethyl (AM) ester suggested that the extracellular Ca 2+ availability is necessary to trigger the response to silver ions and that the intracellular Ca 2+ pool alone is not sufficient for this effect. Altogether, our data suggest that in plant cells the effects of silver ions originate from the primal modification of the internal calcium levels, possibly by their interaction with Ca 2+permeable channels at the plasma membrane.
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