Features of the organization of bread wheat chromosome 5BS based on physical mapping

0488796 - ÚEB 2019 RIV US eng J - Journal Article
Salina, E.A. - Nesterov, V. - Frenkel, Z. - Kiseleva, V. I. - Timonova, E. M. - Magni, F. - Vrána, Jan - Šafář, Jan - Šimková, Hana - Doležel, Jaroslav - Korol, A. - Sergeeva, E.M.
Features of the organization of bread wheat chromosome 5BS based on physical mapping.
BMC Genomics. Roč. 19, FEB 9 (2018), č. článku 80. ISSN 1471-2164. E-ISSN 1471-2164
R&D Projects: GA ČR GBP501/12/G090; GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : Chromosome 5BS * Genetic markers * Hexaploid wheat * Physical mapping * Sequencing * Synteny * Triticum aestivum
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 3.501, year: 2018

Background: The IWGSC strategy for construction of the reference sequence of the bread wheat genome is based on first obtaining physical maps of the individual chromosomes. Our aim is to develop and use the physical map for analysis of the organization of the short arm of wheat chromosome 5B (5BS) which bears a number of agronomically important genes, including genes conferring resistance to fungal diseases. Results: A physical map of the 5BS arm (290 Mbp) was constructed using restriction fingerprinting and LTC software for contig assembly of 43,776 BAC clones. The resulting physical map covered ~ 99% of the 5BS chromosome arm (111 scaffolds, N50 = 3.078 Mb). SSR, ISBP and zipper markers were employed for anchoring the BAC clones, and from these 722 novel markers were developed based on previously obtained data from partial sequencing of 5BS. The markers were mapped using a set of Chinese Spring (CS) deletion lines, and F2 and RICL populations from a cross of CS and CS-5B dicoccoides.
Permanent Link: http://hdl.handle.net/11104/0283333