Heterodera schachtii Tyrosinase-like protein a novel nematode effector modulating plant hormone homeostasis

0488190 - ÚEB 2018 RIV GB eng J - Journal Article
Habash, S. - Radakovic, Z.S. - Vaňková, Radomíra - Siddique, S. - Dobrev, Petre - Gleason, C. - Grundler, F.M.W. - Elashry, A.
Heterodera schachtii Tyrosinase-like protein a novel nematode effector modulating plant hormone homeostasis.
Scientific Reports. Roč. 7, JUL 31 (2017), č. článku 6874. ISSN 2045-2322. E-ISSN 2045-2322
Institutional support: RVO:61389030
Keywords : arabidopsis-thaliana * cyst-nematode * parasitic nematode * transient expression * host plants * sequence * identification * infection * model * transformation
OECD category: Plant sciences, botany
Impact factor: 4.122, year: 2017

The beet cyst nematode Heterodera schachtii causes major yield losses in sugar beet. Understanding the interaction between H. schachtii and its host plant is important for developing a sustainable management system. Nematode effectors play a crucial role in initializing and sustaining successful parasitism. In our study, we identified a gene (Hs-Tyr) encoding a tyrosinase functional domain (PF00264). We describe Hs-Tyr as a novel nematode effector. Hs-Tyr is localized in the nematode esophageal gland. Up-regulation of its expression coincided with the parasitic developmental stages of the nematode. Silencing Hs-Tyr by RNA interference made the treated nematodes less virulent. When RNAi-treated nematodes succeeded in infecting the plant, developing females and their associated syncytial nurse cells were significantly smaller than in control plants. Ectopically expressing the Hs-Tyr effector in Arabidopsis increased plant susceptibility to H. schachtii, but not to the root-knot nematode Meloidogyne incognita. Interestingly, Hs-Tyr in the plant promoted plant growth and changed the root architecture. Additionally, the expression of Hs-Tyr in Arabidopsis caused changes in the homeostasis of several plant hormones especially auxin and the ethylene precursor aminocyclopropane-carboxylic acid.
Permanent Link: http://hdl.handle.net/11104/0282797