A Stepwise Approach for the Synthesis of Folic Acid Conjugates with Protein Kinase Inhibitors

0485773 - ÚEB 2018 RIV US eng J - Journal Article
Krajčovičová, S. - Gucký, Tomáš - Hendrychová, Denisa - Kryštof, Vladimír - Soural, M.
A Stepwise Approach for the Synthesis of Folic Acid Conjugates with Protein Kinase Inhibitors.
Journal of Organic Chemistry. Roč. 82, č. 24 (2017), s. 13530-13541. ISSN 0022-3263. E-ISSN 1520-6904
R&D Projects: GA ČR(CZ) GA15-15264S; GA MŠMT(CZ) LO1304
Institutional support: RVO:61389030
Keywords : CYCLIN-DEPENDENT KINASES * FOLATE RECEPTOR * REGIOSELECTIVE SYNTHESIS
OECD category: Organic chemistry
Impact factor: 4.805, year: 2017

Herein, we report an alternative synthetic approach for selected 2,6,9-trisubstituted purine CDK inhibitor conjugates with folic acid as a drug-delivery system targeting folate receptors. In contrast to the previously reported approaches, the desired conjugates were constructed stepwise using solid-phase synthesis starting from immobilized primary amines. The ability of the prepared conjugates to release the free drug was verified using dithiothreitol (DTT) and glutathione (GSH) as liberating agents. Finally, binding to the folate receptor (FOLR1) overexpressed in a cancer cell line was measured by flow cytometry using a fluorescent imaging probe.
Permanent Link: http://hdl.handle.net/11104/0280716