Evolutionary conserved cysteines function as cis-acting regulators of arabidopsis PIN-FORMED 2 distribution

0485766 - ÚEB 2018 RIV CH eng J - Journal Article
Retzer, Katarzyna - Lacek, Jozef - Skokan, Roman - Del Genio, C. H. - Vosolsobě, S. - Laňková, Martina - Malínská, Kateřina - Konstantinova, N. - Zažímalová, Eva - Napier, R. M. - Petrášek, Jan - Luschnig, C.
Evolutionary conserved cysteines function as cis-acting regulators of arabidopsis PIN-FORMED 2 distribution.
International Journal of Molecular Sciences. Roč. 18, č. 11 (2017), č. článku 2274. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GAP305/11/0797
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
Institutional support: RVO:61389030
Keywords : Arabidopsis * Auxin * Intracellular distribution * PIN proteins * Plasma membrane protein sorting * Protein mobility * Protein modeling * Root phenotype * srrf
OECD category: Plant sciences, botany
Impact factor: 3.687, year: 2017

Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2 C−A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2 C−A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport.
Permanent Link: http://hdl.handle.net/11104/0280709