Subcellular localization of Arabidopsis pathogenesis-related 1 (PR1) protein

0476648 - ÚEB 2018 RIV CH eng J - Journal Article
Pečenková, Tamara - Pleskot, Roman - Žárský, V.
Subcellular localization of Arabidopsis pathogenesis-related 1 (PR1) protein.
International Journal of Molecular Sciences. Roč. 18, č. 4 (2017), č. článku 825. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA15-14886S
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
Institutional support: RVO:61389030
Keywords : cape * mvb * pi(3)p * pr1 * Secretion * Trafficking
OECD category: Cell biology
Impact factor: 3.687, year: 2017

The Arabidopsis thaliana pathogenesis-related 1 (PR1) is an important defense protein, so far it has only been detected in extracellular space and its subcellular sorting and transport remain unexplained. Using a green fluorescent protein (GFP) tagged full length, as well as a C-terminus truncated version of PR1, we observed that when expressed ectopically in Nicotiana benthamiana leaves, PR1 co-localizes only partially with Golgi markers, and much more prominently with the late endosome (LE)/multivesicular body (MVB) FYVE marker. The C-truncated version PR1∆C predominantly localized to the endoplasmic reticulum (ER). The same localizations were found for stable Arabidopsis transformants with expression of PR1 and PR1∆C driven by the native promoter. We conclude that the A. thaliana PR1 (AtPR1) undergoes an unconventional secretion pathway, starting from the C-terminus-dependent sorting from the ER, and utilizing further transportation via phosphatidyl-inositol-3-phosphate (PI(3)P) positive LE/MVB-like vesicles. The homology model of the PR1 structure shows that the cluster of positively charged amino acid residues (arginines 60, 67, 137, and lysine 135) could indeed interact with negatively charged phospholipids of cellular membranes. It remains to be resolved whether Golgi and LE/MVB localization reflects an alternative sorting or trafficking succession, and what the role of lipid interactions in it will be.
Permanent Link: http://hdl.handle.net/11104/0273111