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Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping

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    0476566 - ÚEB 2018 RIV US eng J - Journal Article
    Majka, J. - Ksiazczyk, T. - Kiełbowicz-Matuk, A. - Kopecký, David - Kosmala, A.
    Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping.
    PLoS ONE. Roč. 12, č. 6 (2017), č. článku e0179043. ISSN 1932-6203. E-ISSN 1932-6203
    Institutional support: RVO:61389030
    Keywords : IN-SITU HYBRIDIZATION * RIBOSOMAL-RNA GENES * LOLIUM-MULTIFLORUM
    Subject RIV: EB - Genetics ; Molecular Biology
    OBOR OECD: Plant sciences, botany
    Impact factor: 2.766, year: 2017

    The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyotype remains unrecognized. Only two F. pratensis chromosomes with 35S and 5S rDNA sequences can be easily identified, but its remaining chromosomes have not been distinguished to date. Here, two libraries derived from F. pratensis nuclear DNA with various contents of repetitive DNA sequences were used as sources of molecular probes for fluorescent in situ hybridisation (FISH), a BAC library and a library representing sequences most frequently present in the F. pratensis genome. Using FISH, six groups of DNA sequences were revealed in chromosomes on the basis of their signal position, including dispersed-like sequences, chromosome painting-like sequences, centromeric-like sequences, knob-like sequences, a group without hybridization signals, and single locus-like sequences. The last group was exploited to develop cytogenetic maps of diploid and tetraploid F. pratensis, which are presented here for the first time and provide a remarkable progress in karyotype characterization.
    Permanent Link: http://hdl.handle.net/11104/0273041
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