Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen

0476550 - ÚEB 2018 RIV GB eng J - Journal Article
Dhandapani, P. - Song, J. - Novák, Ondřej - Jameson, P. E.
Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen.
Annals of Botany. Roč. 119, č. 5 (2017), s. 841-852. ISSN 0305-7364. E-ISSN 1095-8290
R&D Projects: GA MŠMT(CZ) LO1204; GA MŠMT LK21306
Institutional support: RVO:61389030
Keywords : Amino acid transporter * Apical dominance * Cell wall invertase * Cytokinin * Cytokinin oxidase/dehydro-genase * Pea * Pisum sativum L. * Rhodococcus fascians * Seed * Sink and source * Sucrose transporter * sweet
OECD category: Plant sciences, botany
Impact factor: 3.646, year: 2017

Background and Aims: Pisum sativum L. (pea) seed is a source of carbohydrate and protein for the developing plant. By studying pea seeds inoculated by the cytokinin-producing bacterium, Rhodococcus fascians, we sought to determine the impact of both an epiphytic (avirulent) strain and a pathogenic strain on source-sink activity within the cotyledons during and following germination.
Methods: Bacterial spread was monitored microscopically, and real-time reverse transcription-quantitative PCR was used to determine the expression of cytokinin biosynthesis, degradation and response regulator gene family members, along with expression of family members of SWEET, SUT, CWINV and AAP genes gene families identified initially in pea by transcriptomic analysis. The endogenous cytokinin content was also determined.
Key Results: The cotyledons infected by the virulent strain remained intact and turned green, while multiple shoots were formed and root growth was reduced. The epiphytic strain had no such marked impact. Isopentenyl adenine was elevated in the cotyledon s infected by the virulent strain. Strong expression of RfIPT, RfLOG and RfCKX was detected in the cotyledons infected by the virulent strain throughout the experiment, with elevated expression also observed for PsSWEET, PsSUT and PsINV gene family members. The epiphytic strain had some impact on the expression of these genes, especially at the later stages of reserve mobilization from the cotyledons.
Conclusions: The pathogenic strain retained the cotyledons as a sink tissue for the pathogen rather than the cotyledon converting completely to a source tissue for the germinating plant. We suggest that the interaction of cytoki-nins, CWINVs and SWEETs may lead to the loss of apical dominance and the appearance of multiple shoots.
Permanent Link: http://hdl.handle.net/11104/0273027