Bridging plant and human radiation response and DNA repair through an in silico approach

0476529 - ÚEB 2018 RIV CH eng J - Journal Article
Nikitaki, Z. - Pavlopoulou, A. - Holá, Marcela - Donà, M. - Michalopoulos, I. - Balestrazzi, A. - Angelis, Karel - Georgakilas, A. G.
Bridging plant and human radiation response and DNA repair through an in silico approach.
Cancers (Basel). Roč. 9, č. 6 (2017), č. článku 65. E-ISSN 2072-6694
R&D Projects: GA ČR GA16-01137S
Institutional support: RVO:61389030
Keywords : Bioinformatics * DNA damage repair * In silico analysis * Ionizing radiation * Plant radiation biodosimeter * Ultraviolet radiation
OECD category: Oncology
Impact factor: 5.326, year: 2017

The mechanisms of response to radiation exposure are conserved in plants and animals. The DNA damage response (DDR) pathways are the predominant molecular pathways activated upon exposure to radiation, both in plants and animals. The conserved features of DDR in plants and animals might facilitate interdisciplinary studies that cross traditional boundaries between animal and plant biology in order to expand the collection of biomarkers currently used for radiation exposure monitoring (REM) in environmental and biomedical settings. Genes implicated in trans-kingdom conserved DDR networks often triggered by ionizing radiation (IR) and UV light are deposited into biological databases. In this study, we have applied an innovative approach utilizing data pertinent to plant and human genes from publicly available databases towards the design of a ‘plant radiation biodosimeter’, that is, a plant and DDR gene-based platform that could serve as a REM reliable biomarker for assessing environmental radiation exposure and associated risk. From our analysis, in addition to REM biomarkers, a significant number of genes, both in human and Arabidopsis thaliana, not yet characterized as DDR, are suggested as possible DNA repair players. Last but not least, we provide an example on the applicability of an Arabidopsis thaliana—based plant system monitoring the role of cancer-related DNA repair genes BRCA1, BARD1 and PARP1 in processing DNA lesions.
Permanent Link: http://hdl.handle.net/11104/0273008