ZnO nanoparticle effects on hormonal pools in Arabidopsis thaliana

0476522 - ÚEB 2018 RIV NL eng J - Journal Article
Vaňková, Radomíra - Landa, Přemysl - Podlipná, Radka - Dobrev, Petre - Přerostová, Sylva - Langhansová, Lenka - Gaudinová, Alena - Moťková, Kateřina - Knirsch, Vojtěch - Vaněk, Tomáš
ZnO nanoparticle effects on hormonal pools in Arabidopsis thaliana.
Science of the Total Environment. Roč. 593, SEP 1 (2017), s. 535-542. ISSN 0048-9697. E-ISSN 1879-1026
R&D Projects: GA MŠMT LD14125; GA MŠMT LD14120; GA MŠMT 8G15003
Institutional support: RVO:61389030
Keywords : Abscisic acid * Cytokinin * Plant hormone * ZnO nanoparticle
OECD category: Plant sciences, botany
Impact factor: 4.610, year: 2017

At present, nanoparticles have been more and more used in a wide range of areas. However, very little is known about the mechanisms of their impact on plants, as both positive and negative effects have been reported. As plant interactions with the environment are mediated by plant hormones, complex phytohormone analysis has been performed in order to characterize the effect of ZnO nanoparticles (mean size 30 nm, concentration range 0.16–100 mg L − 1 ) on Arabidopsis thaliana plants. Taking into account that plant hormones exhibit high tissue-specificity as well as an intensive cross-talk in the regulation of growth and development as well as defense, plant responses were followed by determination of the content of five main phytohormones (cytokinins, auxins, abscisic acid, salicylic acid and jasmonic acid) in apices, leaves and roots. Increasing nanoparticle concentration was associated with gradually suppressed biosynthesis of the growth promoting hormones cytokinins and auxins in shoot apical meristems (apices). In contrast, cis-zeatin, a cytokinin associated with stress responses, was elevated by 280% and 590% upon exposure to nanoparticle concentrations 20 and 100 mg L − 1 , respectively, in roots. Higher ZnO nanoparticle doses resulted in up-regulation of the stress hormone abscisic acid, mainly in apices and leaves. In case of salicylic acid, stimulation was found in leaves and roots. The other stress hormone jasmonic acid (as well as its active metabolite jasmonate isoleucine) was suppressed at the presence of nanoparticles. The earliest response to nanoparticles, associated with down-regulation of growth as well as of cytokinins and auxins, was observed in apices. At higher dose, up-regulation of abscisic acid, was detected. This increase, together with elevation of the other stress hormone salicylic acid, indicates that plants sense nanoparticles as severe stress. Gradual accumulation of cis-zeatin in roots may contribute to relatively higher stress resistance of this tissue.
Permanent Link: http://hdl.handle.net/11104/0273001