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Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

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    0475872 - ÚEB 2018 RIV US eng J - Journal Article
    Omidvar, Vahid - Mohorianu, I. - Dalmay, T. - Zheng, Y. - Fei, Z. - Pucci, A. - Mazzucato, E. - Večeřová, R. - Sedlářová, M. - Fellner, Martin
    Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant.
    PLoS ONE. Roč. 12, č. 2 (2017), č. článku e0170715. E-ISSN 1932-6203
    R&D Projects: GA MŠk(CZ) LO1204
    Institutional support: RVO:61389030
    Keywords : tapetum-degeneration-retardation * male reproductive development * early anther development * differential expression * lycopersicon-esculentum * stamenless-2 mutant * abscisic-acid * arabidopsis-thaliana * brassica-napus * cell-wall
    Subject RIV: EB - Genetics ; Molecular Biology
    OBOR OECD: Plant sciences, botany
    Impact factor: 2.766, year: 2017

    The 78-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 78-1, transcriptomic profiles of the 78-1 malesterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 upregulated and 636 downregulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 78-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 78-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 78-1 anthers. The present study provides insights into the transcriptome of the 78-1 mutant. We identified several genes with altered expression level in 78-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.
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