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Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor

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    0474824 - ÚEB 2018 RIV GB eng J - Journal Article
    Langhansová, Lenka - Landa, Přemysl - Kutil, Zsófia - Tauchen, Jan - Maršík, Petr - Rezek, Jan - Lou, J.D. - Yun, Z.L. - Vaněk, Tomáš
    Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor.
    Food and Agricultural Immunology. Roč. 28, č. 2 (2017), s. 343-353. ISSN 0954-0105. E-ISSN 1465-3443
    R&D Projects: GA MŠk LH12165; GA ČR(CZ) GA16-07193S
    Grant - others:OPPK(CZ) CZ.2.16/3.1.00/24014
    Institutional support: RVO:61389030
    Keywords : in-vitro * antiinflammatory activity * zucc. leaves * 5-lipoxygenase * sieb. * cyclooxygenase * constituents * antioxidants * myricitrin * metabolism * Myrica rubra * anti-inflammatory * cyclooxygenase 1 * cyclooxygenase 2 * 5-lipoxygenase
    Subject RIV: FR - Pharmacology ; Medidal Chemistry
    OBOR OECD: Medicinal chemistry
    Impact factor: 2.568, year: 2017

    Myrica rubra Sieb. et Zucc. is a valuable fruit tree that is used in Chinese, Japanese and Taiwanese traditional medicine. We investigated the anti-inflammatory activity of M. rubra leaves extracted with four different solvents. Total phenolics were determined using the Folin-Ciocalteu method. Extracts were investigated for their inhibitory activity toward the pro-inflammatory enzymes cyclooxygenase-1 and2 (COX-1, COX-2) and 5-lipoxygenase (5-LOX). The ethanol extract of M. rubra leaves demonstrated a strong inhibition of prostaglandin E-2 (PGE(2)) biosynthesis catalyzed by both COX-1 (93.42%) and COX-2 (75.71%) and leukotriene B-4 (LTB4) formation catalyzed by 5-LOX (82.72%). Further we identified selective COX-1 inhibition by the n-butanol and aqueous fractions of the ethanol extract (with an IC50 for COX-1 inhibition of 1.07 and 0.71 mu gmL(-1) (,) respectively) and dual 5-LOX/COX inhibition by the ethyl acetate fraction (with an IC50 of 3.29 for COX-1, 2.54 for COX-2 and 8.30 mu gmL(-1) for 5-LOX).
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