Number of the records: 1  

Flow Analysis and Sorting of Plant Chromosomes

  1. 1.
    0471598 - ÚEB 2017 RIV US eng J - Journal Article
    Vrána, Jan - Cápal, Petr - Šimková, Hana - Karafiátová, Miroslava - Čížková, Jana - Doležel, Jaroslav
    Flow Analysis and Sorting of Plant Chromosomes.
    Current protocols in cytometry. Roč. 78, Oct 10 (2016), 5.3.1-5.3.43. ISSN 1934-9300
    R&D Projects: GA MŠk(CZ) LO1204
    Institutional support: RVO:61389030
    Keywords : cell cycle synchronization * chromosome genomics * chromosome isolation
    Subject RIV: EB - Genetics ; Molecular Biology

    Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed.
    Permanent Link:

    2016_Vrana_Current Protocols in Cytometry_1-43.pdf0504 KBOtheropen-access
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.