Markers of Lipid Oxidative Damage in the Exhaled Breath Condensate of Nano TiO2 Production Workers.

0470937 - ÚCHP 2017 RIV GB eng J - Journal Article
Pelclová, D. - Ždímal, Vladimír - Kačer, P. - Zíková, Naděžda - Komarc, M. - Fenclová, Z. - Vlčková, Š. - Schwarz, Jaroslav - Makeš, Otakar - Syslová, K. - Navrátil, Tomáš - Turci, F. - Corazzari, I. - Zakharov, S. - Bello, D.
Markers of Lipid Oxidative Damage in the Exhaled Breath Condensate of Nano TiO2 Production Workers.
Nanotoxicology. Roč. 11, č. 1 (2017), s. 52-63. ISSN 1743-5390. E-ISSN 1743-5404
R&D Projects: GA ČR(CZ) GBP503/12/G147
Institutional support: RVO:67985858 ; RVO:61388955
Keywords : exhaled breath condensate * aldehydes * oxidative stress * occupational exposure * monitoring
OECD category: Public and environmental health; Physical chemistry (UFCH-W)
Impact factor: 5.811, year: 2017

Nanoscale titanium dioxide (nanoTiO2) is a commercially important nanomaterial. Animal studies have documented lung injury and inflammation, oxidative stress, cytotoxicity and genotoxicity. Yet human health data are scarce and quantitative risk assessments and biomonitoring of exposure are lacking. NanoTiO2 is classified by IARC as a group 2B, possible human carcinogen. In our earlier studies we documented an increase in markers of inflammation, as well as DNA and protein oxidative damage, in exhaled breath condensate (EBC) of workers exposed nanoTiO2. This study focuses on biomarkers of lipid oxidation. Several established lipid oxidative markers (malondialdehyde, 4-hydroxy-trans-hexenal, 4-hydroxy-trans-nonenal, 8-isoProstaglandin F2α and aldehydes C6-C12) were studied in EBC and urine of 34 workers and 45 comparable controls. The median particle number concentration in the production line ranged from 1.98x104 to 2.32x104 particles/cm3 with ∼80% of the particles <100 nm in diameter. Mass concentration varied between 0.40-0.65 mg/m3. All 11 markers of lipid oxidation were elevated in production workers relative to the controls (p < 0.001). A significant dose-dependent association was found between exposure to TiO2 and markers of lipid oxidation in the EBC. These markers were not elevated in the urine samples. Lipid oxidation in the EBC of workers exposed to (nano)TiO2 complements our earlier findings on DNA and protein damage. These results are consistent with the oxidative stress hypothesis and suggest lung injury at the molecular level. Further studies should focus on clinical markers of potential disease progression. EBC has reemerged as a sensitive technique for non-invasive monitoring of workers exposed to engineered nanoparticles.
Permanent Link: http://hdl.handle.net/11104/0270483