Rapid gene isolation in barley and wheat by mutant chromosome sequencing

0465513 - ÚEB 2017 RIV US eng J - Journal Article
Sanchez-Martin, J. - Steuernagel, B. - Ghosh, S. - Herren, G. - Hurni, S. - Adamski, N. - Vrána, Jan - Kubaláková, Marie - Krattinger, S.G. - Wicker, T. - Doležel, Jaroslav - Keller, B. - Wulff, B. B. H.
Rapid gene isolation in barley and wheat by mutant chromosome sequencing.
Genome Biology. Roč. 17, OCT 31 (2016), č. článku 221. ISSN 1465-6906
R&D Projects: GA ČR GBP501/12/G090; GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : induced mutations * mitotic chromosomes * confers resistance * exome capture * genome * identification * evolution * pathogens * hordeum * MutChromSeq * Gene cloning * Mutational genomics * Chromosome flow sorting * Triticeae * Wheat * Barley
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 11.313, year: 2015

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.
Permanent Link: http://hdl.handle.net/11104/0264045