Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus

0464107 - ÚEB 2017 RIV US eng J - Journal Article
Nováková, Miroslava - Kim, P.D. - Šašek, Vladimír - Burketová, Lenka - Jindřichová, Barbora - Šantrůček, J. - Valentová, O.
Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus.
Biotechnology Progress. Roč. 32, č. 4 (2016), s. 918-928. ISSN 8756-7938. E-ISSN 1520-6033
R&D Projects: GA ČR GA522/08/1581; GA MZe QH81201; GA MŠMT LD14093
Institutional support: RVO:61389030
Keywords : elicitor * Brassica napus * Leptosphaeria maculans
Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection
Impact factor: 1.986, year: 2016

The Dothideomycete Leptosphaeria maculans, a worldwide fungal pathogen of oilseed rape (Brassica napus), secretes a broad spectrum of molecules into the cultivation medium during growth in vitro. Here, candidate elicitor molecules, which induce resistance in B. napus to L. maculans, were identified in the cultivation medium. The elicitation activity was indicated by increased transcription of pathogenesis-related gene 1 (PR1) and enhanced resistance of B. napus plants to the invasion of L. maculans. The elicitation activity was significantly lowered when the cultivation medium was heated to 80 degrees C. Active components were further characterized by specific cleavage with the proteolytic enzymes trypsin and proteinase K and with glycosidases -amylase and -glucanase. The elicitor activity was eliminated by proteolytic digestion while glycosidases had no effect. The filtered medium was fractionated by either ion-exchange chromatography or isoelectric focusing. Mass spectrometry analysis of the most active fractions obtained by both separation procedures revealed predominantly enzymes that can be involved in the degradation of plant cell wall polysaccharides. This is the first study searching for L. maculans-specific secreted elicitors with a potential to be used as defense-activating agents in the protection of B. napus against L. maculans in agriculture.
Permanent Link: http://hdl.handle.net/11104/0263150