The Recently Identified Isoleucine Conjugate of cis-12-Oxo-Phytodienoic Acid Is Partially Active in cis-12-Oxo-Phytodienoic Acid-Specific Gene Expression of Arabidopsis thaliana

0464093 - ÚEB 2017 RIV US eng J - Journal Article
Arnold, M.D. - Gruber, C. - Floková, Kristýna - Miersch, Otto - Strnad, Miroslav - Novák, Ondřej - Wasternack, Claus - Hause, B.
The Recently Identified Isoleucine Conjugate of cis-12-Oxo-Phytodienoic Acid Is Partially Active in cis-12-Oxo-Phytodienoic Acid-Specific Gene Expression of Arabidopsis thaliana.
PLoS ONE. Roč. 11, č. 9 (2016), e0162829. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA14-34792S; GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : ALLENE-OXIDE-CYCLASE * 12-OXO-PHYTODIENOIC ACID * JASMONIC ACID
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.806, year: 2016

Oxylipins of the jasmonate family are active as signals in plant responses to biotic and abiotic stresses as well as in development. Jasmonic acid (JA), its precursor cis-12-oxo-phytodienoic acid (OPDA) and the isoleucine conjugate of JA (JA-Ile) are the most prominent members. OPDA and JA-Ile have individual signalling properties in several processes and differ in their pattern of gene expression. JA-Ile, but not OPDA, is perceived by the SCFCOI1-JAZ co-receptor complex. There are, however, numerous processes and genes specifically induced by OPDA. The recently identified OPDA-Ile suggests that OPDA specific responses might be mediated upon formation of OPDA-Ile. Here, we tested OPDA-Ile-induced gene expression in wild type and JA-deficient, JA-insensitive and JA-Ile-deficient mutant background. Tests on putative conversion of OPDA-Ile during treatments revealed only negligible conversion. Expression of two OPDA-inducible genes, GRX480 and ZAT10, by OPDA-Ile could be detected in a JA-independent manner in Arabidopsis seedlings but less in flowering plants. The data suggest a bioactivity in planta of OPDA-Ile.
Permanent Link: http://hdl.handle.net/11104/0263140