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Solvatochromic fluorene-linked nucleoside and DNA as color-changing fluorescent probes for sensing interactions
- 1.0463825 - ÚOCHB 2017 RIV GB eng J - Journal Article
Dziuba, Dmytro - Pospíšil, Petr - Matyašovský, Ján - Brynda, Jiří - Nachtigallová, Dana - Rulíšek, Lubomír - Pohl, Radek - Hof, Martin - Hocek, Michal
Solvatochromic fluorene-linked nucleoside and DNA as color-changing fluorescent probes for sensing interactions.
Chemical Science. Roč. 7, č. 9 (2016), s. 5775-5785. ISSN 2041-6520. E-ISSN 2041-6539
R&D Projects: GA ČR GBP206/12/G151; GA ČR(CZ) GC14-03141J; GA ČR(CZ) GA14-31419S; GA MŠMT(CZ) LO1304
EU Projects: European Commission(XE) 642023 - ClickGene
Institutional support: RVO:61388963 ; RVO:61388955
Keywords : environmentally sensitive fluorescent * molecular dynamics simulations * oligonucleotides
Subject RIV: CC - Organic Chemistry; CF - Physical ; Theoretical Chemistry (UFCH-W)
Impact factor: 8.668, year: 2016
http://pubs.rsc.org/en/content/articlepdf/2016/sc/c6sc02548j
A nucleoside bearing a solvatochromic push-pull fluorene fluorophore (dC(FL)) was designed and synthesized by the Sonogashira coupling of alkyne-linked fluorene 8 with 5-iodo-2'-deoxycytidine. The fluorene building block 8 and labeled nucleoside dC(FL) exerted bright fluorescence with significant solvatochromic effect providing emission maxima ranging from 421 to 544 nm and high quantum yields even in highly polar solvents, including water. The solvatochromism of 8 was studied by DFT and ADC(2) calculations to show that, depending on the polarity of the solvent, emission either from the planar or the twisted conformation of the excited state can occur. The nucleoside was converted to its triphosphate variant dC(FL)TP which was found to be a good substrate for DNA polymerases suitable for the enzymatic synthesis of oligonucleotide or DNA probes by primer extension or PCR. The fluorene-linked DNA can be used as fluorescent probes for DNA-protein (p53) or DNA-lipid interactions, exerting significant color changes visible even to the naked eye. They also appear to be suitable for time-dependent fluorescence shift studies on DNA, yielding information on DNA hydration and dynamics.
Permanent Link: http://hdl.handle.net/11104/0262923
File Download Size Commentary Version Access 0463825.pdf 3 1 MB Publisher’s postprint open-access
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