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PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

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    0462665 - ÚEB 2017 RIV GB eng J - Journal Article
    Simon, S. - Skůpa, Petr - Viaene, T. - Zwiewka, M. - Tejos, R. - Klíma, Petr - Čarná, Mária - Rolčík, J. - De Rycke, R. - Moreno, I. - Dobrev, Petre - Orellana, A. - Zažímalová, Eva - Friml, J.
    PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis.
    New Phytologist. Roč. 211, č. 1 (2016), s. 65-74. ISSN 0028-646X. E-ISSN 1469-8137
    R&D Projects: GA MŠk(CZ) ED1.1.00/02.0068; GA ČR(CZ) GA16-10948S
    Institutional support: RVO:61389030
    Keywords : auxin * endoplasmic reticulum (ER) * lateral root
    Subject RIV: ED - Physiology
    Impact factor: 7.330, year: 2016

    Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate.

    In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles.

    The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes.

    These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
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