Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro

0461726 - ÚEB 2017 RIV GB eng J - Journal Article
Kubatka, P. - Kello, M. - Kajo, K. - Kruzliak, P. - Výbohová, D. - Šmejkal, K. - Maršík, Petr - Zullini, A. - Gönciová, G. - Mojžíš, J. - Kapinová, A. - Murin, R. - Péč, M. - Adamkov, M. - Przygodzki, R.M.
Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro.
Nutrition and Cancer-An International Journal. Roč. 68, č. 4 (2016), s. 611-621. ISSN 0163-5581. E-ISSN 1532-7914
Institutional support: RVO:61389030
Keywords : DIETARY FLAVONOID INTAKE * CELL-CYCLE ARREST * ANTIOXIDANT ACTIVITY
Subject RIV: ED - Physiology
Impact factor: 2.447, year: 2016

The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.
Permanent Link: http://hdl.handle.net/11104/0261312