Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

0461616 - ÚEB 2017 RIV US eng J - Journal Article
Hafidh, Said - Potěšil, D. - Fíla, Jan - Čapková, Věra - Zdráhal, Z. - Honys, David
Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization.
Genome Biology. Roč. 17, MAY 3 (2016), č. článku 81. ISSN 1465-6906
R&D Projects: GA ČR GA15-22720S; GA ČR(CZ) GA14-32292S; GA ČR(CZ) GA15-16050S; GA MŠMT(CZ) LD14109; GA MŠMT(CZ) LM2015043; GA MŠMT(CZ) ED1.1.00/02.0068; GA MŠMT(CZ) LQ1601
Institutional support: RVO:61389030
Keywords : Protein secretion * Pollen tube guidance * Cell-cell signaling
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 11.313, year: 2015

We performed genome-wide quantitative liquid chromatography-tandem mass spectrometry analysis of a pistil-stimulated pollen tube secretome and identified 801 pollen tube-secreted proteins. Interestingly, in silico analysis reveals that the pollen tube secretome is dominated by proteins that are secreted unconventionally, representing 57 % of the total secretome. In support, we show that an unconventionally secreted protein, translationally controlled tumor protein, is secreted to the apoplast. Remarkably, we discovered that this protein could be secreted by infiltrating through the initial phases of the conventional secretory pathway and could reach the apoplast via exosomes, as demonstrated by co-localization with Oleisin1 exosome marker. We demonstrate that translationally controlled tumor protein-knockdown Arabidopsis thaliana plants produce pollen tubes that navigate poorly to the target ovule and that the mutant allele is poorly transmitted through the male. Further, we show that regulators of the endoplasmic reticulum-trans-Golgi network protein secretory pathway control secretion of Nicotiana tabacum Pollen tube-secreted cysteine-rich protein 2 and Lorelei-like GPI-anchor protein 3 and that a regulator of endoplasmic reticulum-trans-Golgi protein translocation is essential for pollen tube growth, pollen tube guidance and ovule-targeting competence.
Permanent Link: http://hdl.handle.net/11104/0261222