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Repetitive Inductions of Bioluminescence of Pseudomonas putida TVA8 Immobilized by Adsorption on Optical Fibre.

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    0461501 - ÚCHP 2017 RIV SK eng J - Journal Article
    Zajíc, J. - Bittner, M. - Brányik, T. - Solovyev, Andrey - Šabata, Stanislav - Kuncová, Gabriela - Pospíšilová, M.
    Repetitive Inductions of Bioluminescence of Pseudomonas putida TVA8 Immobilized by Adsorption on Optical Fibre.
    Chemical Papers. Roč. 70, č. 7 (2016), s. 877-887. ISSN 0366-6352
    Institutional support: RVO:67985858
    Keywords : whole-cell biosensor * bioluminiscent bioreporter * optical fibre sensor
    Subject RIV: CC - Organic Chemistry
    Impact factor: 1.258, year: 2016

    Physico-chemical models of the interactions of cells with solid surfaces, which use contact angles and zeta potentials, indicated more facile adsorption of cells of Pseudomonas putida TVA8 on the quartz surface after its treatment with 3-aminopropyltriethoxysilane (APTES). A whole-cell optical fibre sensor of toluene was prepared by the adsorption of P. putida TVA8, bacteria producing light in contact with toluene on the wider end of APTES-treated quartz tapered optical fibre. The results of the measurements of luminescence from both sides of the layer of adsorbed cells were compared. Over the 135 days trial, the fibre biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of bioluminescence gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of bioluminescence induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene.
    Permanent Link: http://hdl.handle.net/11104/0261135

     
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