Effects of BP-14, a novel cyclin-dependent kinase inhibitor, on anaplastic thyroid cancer cells

0459800 - ÚEB 2017 RIV GR eng J - Journal Article
Allegri, L. - Baldan, F. - Mio, F. - Puppin, C. - Russo, D. - Kryštof, Vladimír - Damante, G.
Effects of BP-14, a novel cyclin-dependent kinase inhibitor, on anaplastic thyroid cancer cells.
Oncology Reports. Roč. 35, č. 4 (2016), s. 2413-2418. ISSN 1021-335X. E-ISSN 1791-2431
R&D Projects: GA ČR(CZ) GA15-15264S
Institutional support: RVO:61389030
Keywords : mTOR * thyroid cancer * cell proliferation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.662, year: 2016

Anaplastic thyroid carcinoma (ATC) is an extremely aggressive human malignancy characterized by a marked degree of invasiveness, absense of features of thyroid differentiation and resistance to current medical treatment. It is well known that ATCs are characterized by deregulation of genes related to cell cycle regulation, i.e., cyclin-dependent kinases (CDKs) and endogenous cyclin-dependent kinase inhibitors (CDKIs). Therefore, in the present study, the effect of a novel exogenous cyclin-dependent kinase inhibitor, BP-14, was investigated in three human ATC cell lines. The ATC-derived cell lines FRO, SW1736 and 8505C were treated with BP-14 alone or in combination with the mTOR inhibitor everolimus. In all ATC cell lines, treatment with BP-14 decreased cell viability and, in two of them, BP-14 modified expression of genes involved in epithelial-mesenchymal transition. Thus, our data indicate that BP-14 is a potential new compound effective against ATC. Combined treatment with BP-14 and the mTOR inhibitor everolimus had a strong synergistic effect on cell viability in all three cell lines, suggesting that the combined used of CDK and mTOR inhibitors may be a useful strategy for ATC treatment.
Permanent Link: http://hdl.handle.net/11104/0259958