Cytokinin profiles in ex vitro acclimatized Eucomis autumnalis plants pre-treated with smoke-derived karrikinolide

0459317 - ÚEB 2017 RIV DE eng J - Journal Article
Aremu, A.O. - Plačková, Lenka - Novák, Ondřej - Stirk, W. A. - Doležal, Karel - van Staden, J.
Cytokinin profiles in ex vitro acclimatized Eucomis autumnalis plants pre-treated with smoke-derived karrikinolide.
Plant Cell Reports. Roč. 35, č. 1 (2016), s. 227-238. ISSN 0721-7714. E-ISSN 1432-203X
R&D Projects: GA MŠMT(CZ) LO1204; GA MŠMT LK21306
Institutional support: RVO:61389030
Keywords : Acclimatization * Asparagaceae * Conservation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.869, year: 2016

Karrikinolide (KAR(1)) which is derived from smoke-water (SW) is involved in some physiological aspects in the life-cycle of plants. This suggests a potential influence on the endogenous pool (quantity and quality) of phytohormones such as cytokinins (CKs). In the current study, the effect of SW (1: 500; 1: 1000; 1: 1500 v/v dilutions) and KAR(1) (10(-7); 10(-8); 10(-9) M) applied during micropropagation of Eucomis autumnalis subspecies autumnalis on the ex vitro growth and CKs after 4 months post-flask duration was evaluated. The interactions of SW and KAR(1) with benzyladenine (BA), a-naphthaleneacetic acid (NAA) or BA+NAA were also assessed. Plants treated with SW (1:500) and KAR(1) (10(-8) M) demonstrated superior growth in terms of the rooting, leaf and bulb sizes and fresh biomass than the control and plants treated with BA and BA+NAA. However, plant growth was generally inhibited with either SW (1: 500) or KAR(1) (10(-8) M) and BA when compared to BA (alone) treatment. Relative to NAA treatment, the presence of KAR(1) (10(-7) M) with NAA significantly increased the leaf area and fresh biomass. Both SW and KAR(1)-treated plants accumulated more total CKs, mainly isoprenoid-type than the control and NAA-treated plants. The highest CK content was also accumulated in SW (1:500) with BA+NAA treatments. Similar stimulatory effects were observed with increasing concentrations of KAR(1) and BA. The current findings establish that SW and KAR(1) exert significant influence on the endogenous CK pools. However, the better growth of plants treated with SW and KAR(1) treatments was not exclusively related to the endogenous CKs.
Permanent Link: http://hdl.handle.net/11104/0259530