Aluminum ions alter the function of non-specific phospholipase C through the changes in plasma membrane physical properties

0451392 - ÚEB 2016 RIV US eng J - Journal Article
Pejchar, Přemysl - Martinec, Jan
Aluminum ions alter the function of non-specific phospholipase C through the changes in plasma membrane physical properties.
Plant Signaling & Behavior. Roč. 10, č. 6 (2015), e1031938. ISSN 1559-2316. E-ISSN 1559-2324
R&D Projects: GA ČR(CZ) GPP501/12/P950; GA ČR(CZ) GAP501/12/1942; GA ČR GA13-19073S
Institutional support: RVO:61389030
Keywords : aluminum toxicity * Arabidopsis thaliana * benzyl alcohol
Subject RIV: ED - Physiology

The first indication of the aluminum (Al) toxicity in plants growing in acidic soils is the cessation of root growth, but the detailed mechanism of Al effect is unknown. Here we examined the impact of Al stress on the activity of non-specific phospholipase C (NPC) in the connection with the processes related to the plasma membrane using fluorescently labeled phosphatidylcholine. We observed a rapid and significant decrease of labeled diacylglycerol (DAG), product of NPC activity, in Arabidopsis seedlings treated with AlCl3. Interestingly, an application of the membrane fluidizer, benzyl alcohol, restored the level of DAG during Al treatment. Our observations suggest that the activity of NPC is affected by Al-induced changes in plasma membrane physical properties.
Permanent Link: http://hdl.handle.net/11104/0252567