Number of the records: 1  

Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

  1. 1.
    0451388 - ÚEB 2016 RIV CH eng J - Journal Article
    Krčková, Zuzana - Brouzdová, Jitka - Daněk, Michal - Kocourková, Daniela - Rainteau, D. - Ruelland, E. - Valentová, O. - Pejchar, Přemysl - Martinec, Jan
    Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress.
    Frontiers in Plant Science. Roč. 6, NOV 4 (2015), s. 928. ISSN 1664-462X. E-ISSN 1664-462X
    R&D Projects: GA ČR(CZ) GAP501/12/1942
    Institutional support: RVO:61389030
    Keywords : Arabidopsis thaliana * Diacylglycerol * Heat stress
    Subject RIV: ED - Physiology
    Impact factor: 4.495, year: 2015

    The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
    Permanent Link: http://hdl.handle.net/11104/0252550

     
    FileDownloadSizeCommentaryVersionAccess
    2015_Krckova_FRONTIERS IN PLANT SCIENCE_928.pdf32.5 MBOtheropen-access
     
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.