Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication

0449202 - BÚ 2016 RIV US eng J - Journal Article
Trávníček, Pavel - Ponert, J. - Urfus, Tomáš - Jersáková, Jana - Vrána, Jan - Hřibová, Eva - Doležel, Jaroslav - Suda, Jan
Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication.
Cytometry. Part A. Roč. 87, č. 10 (2015), s. 958-966. ISSN 1552-4922. E-ISSN 1552-4930
R&D Projects: GA ČR GAP506/12/1320
Institutional support: RVO:67985939 ; RVO:67179843 ; RVO:61389030
Keywords : flow cytometry * genome size * hyporeduplication
Subject RIV: EF - Botanics; EF - Botanics (UEB-Q); EH - Ecology, Behaviour (UEK-B)
Impact factor: 3.181, year: 2015

We analyzed six different tissue types in 48 orchid species belonging to all recognized subfamilies. Although traditionally used leaves may provide incorrect C-values, particularly in species with progressively partial endoreplication, young ovaries and pollinaria consistently yield 2C and 1C peaks of their G1-phase nuclei, respectively, and are, therefore, the most suitable parts for genome size studies in orchids. We also provide new DNA C-values for 22 orchid genera and 42 species.
Permanent Link: http://hdl.handle.net/11104/0251050