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Identification of phospholipase D genes in Brassica napus and their transcription after phytohormone treatment and pathogen infection

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    0447327 - ÚEB 2016 RIV NL eng J - Journal Article
    Janda, Martin - Ježková, Lucie - Nováková, Miroslava - Valentová, O. - Burketová, Lenka - Šašek, Vladimír
    Identification of phospholipase D genes in Brassica napus and their transcription after phytohormone treatment and pathogen infection.
    Biologia Plantarum. Roč. 59, č. 3 (2015), s. 581-590. ISSN 0006-3134. E-ISSN 1573-8264
    R&D Projects: GA ČR GA522/08/1581; GA ČR(CZ) GAP501/11/1654
    Institutional support: RVO:61389030
    Keywords : benzothiadiazole * Leptosphaeria maculans * methyl jasmonate
    Subject RIV: ED - Physiology
    Impact factor: 1.665, year: 2015

    Phospholipase D (PLD) and its product phosphatidic acid are now considered to be one of the key elements of numerous physiological processes in plants including the salicylic acid signalling pathway. The presented study investigates the transcriptional regulation of Brassica napus PLDs following treatments with defense-related stimuli. We cloned eight B. napus genes encoding members of PLD beta, gamma, and delta isoforms and performed phylogenetic analysis with its ancestor species Brassica rapa and Brassica oleracea, and with the model plant Arabidopsis thaliana. Transcription of the identified genes was monitored after treatment with benzothiadiazole (BTH), methyl jasmonate (MeJA), bacterial elicitor flg22, wounding, and after infection with fungal pathogens Sclerotinia sclerotiorum and Leptosphaeria maculans. Most of the genes responded specifically to a particular treatment. Remarkably the genes encoding the PLD gamma and PLD beta isoforms were up-regulated by stimuli associated with the salicylic acid signalling pathway. The generality of this finding was confirmed by the analysis of public transcriptional data from Arabidopsis thaliana.
    Permanent Link: http://hdl.handle.net/11104/0249210

     
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