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The influence of the quinone antioxidants tert-butylhydroquinone and 2,5-di-tert-butylhydroquinone on the arachidonic acid metabolism in vitro
- 1.0446709 - ÚEB 2016 RIV GB eng J - Journal Article
Kutil, Zsófia - Kvasnicová, Marie - Temml, V. - Schuster, D. - Vaněk, Tomáš - Fernandez, E. - Malík, J. - Landa, Přemysl
The influence of the quinone antioxidants tert-butylhydroquinone and 2,5-di-tert-butylhydroquinone on the arachidonic acid metabolism in vitro.
Food and Agricultural Immunology. Roč. 26, č. 4 (2015), s. 504-511. ISSN 0954-0105. E-ISSN 1465-3443
Institutional support: RVO:61389030
Keywords : food additives * tert-butylhydroquinone * cyclooxygenase
Subject RIV: CE - Biochemistry
Impact factor: 1.548, year: 2015
The aim of the present study was to elucidate the effect of the food additive tert-butylhydroquinone (TBHQ), its metabolite tert-butylbenzoquinone (TBQ), the indirect food additive 2,5-di-tert-butylhydroquinone (DTBHQ) and five other structurally related quinones on the enzymatic activity of cyclooxygenase-1 and -2 (COX-1, -2) and 5-lipoxygenase (5-LO). In a study conducted on purified enzymes TBHQ affected neither COX-1 nor COX-2 activity, but inhibited 5-LO in human neutrophils with an IC50 of 13.5 mu M. In contrast, DTBHQ regulated the activity of 5-LO as well as the activity of COX-2 (IC50 = 1.8 and 14.1 for 5-LO and COX-2, respectively). The in silico studies on DTBHQ indicated interactions with the catalytic iron of 5-LO and possible hydrogen bond interactions with His367 or His372. These data suggest that TBHQ and DTBHQ influence arachidonic acid (AA) metabolism in biologically relevant concentrations in vitro and should be further studied for their possible effect on the AA pathway in vivo.
Permanent Link: http://hdl.handle.net/11104/0248679
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