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pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes

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    0446475 - ÚIACH 2016 RIV DE eng J - Journal Article
    Hanneken, M. - Šlais, Karel - König, S.
    pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes.
    Data in Brief. Roč. 3, JUN (2015), s. 221-228. ISSN 2352-3409
    Institutional support: RVO:68081715
    Keywords : 2D-PAGE * CoFGE * Gel electrophoresis
    OECD category: Analytical chemistry
    Method of publishing: Open access
    http://www.sciencedirect.com/science/article/pii/S2352340915000414

    Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.
    Permanent Link: http://hdl.handle.net/11104/0248455

     
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