Endogenous Abscisic Acid Promotes Hypocotyl Growth and Affects Endoreduplication during Dark-Induced Growth in Tomato (Solanum lycopersicum L.)

0446265 - ÚEB 2016 RIV US eng J - Journal Article
Humplík, Jan - Bergougnoux, V. - Jandová, M. - Šimura, Jan - Pěnčík, Aleš - Tomanec, O. - Rolčík, Jakub - Novák, Ondřej - Fellner, Martin
Endogenous Abscisic Acid Promotes Hypocotyl Growth and Affects Endoreduplication during Dark-Induced Growth in Tomato (Solanum lycopersicum L.).
PLoS ONE. Roč. 10, č. 2 (2015). ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : DEPENDENT KINASE INHIBITOR * DEFICIENT MUTANT SITIENS * ARABIDOPSIS-THALIANA
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.057, year: 2015

Dark-induced growth (skotomorphogenesis) is primarily characterized by rapid elongation of the hypocotyl. We have studied the role of abscisic acid (ABA) during the development of young tomato (Solanum lycopersicum L.) seedlings. We observed that ABA deficiency caused a reduction in hypocotyl growth at the level of cell elongation and that the growth in ABA-deficient plants could be improved by treatment with exogenous ABA, through which the plants show a concentration dependent response. In addition, ABA accumulated in dark-grown tomato seedlings that grew rapidly, whereas seedlings grown under blue light exhibited low growth rates and accumulated less ABA. We demonstrated that ABA promotes DNA endoreduplication by enhancing the expression of the genes encoding inhibitors of cyclin-dependent kinases SIKRP1 and SIKRP3 and by reducing cytokinin levels. These data were supported by the expression analysis of the genes which encode enzymes involved in ABA and CK metabolism. Our results show that ABA is essential for the process of hypocotyl elongation and that appropriate control of the endogenous level of ABA is required in order to drive the growth of etiolated seedlings.
Permanent Link: http://hdl.handle.net/11104/0248247