Cytokinin levels and signaling respond to wounding and the perception of herbivore elicitors in Nicotiana attenuata

0446188 - ÚEB 2016 RIV GB eng J - Journal Article
Schäfer, M. - Meza-Canales, I.D. - Navarro-Quezada, A. - Brütting, C. - Vaňková, Radomíra - Baldwin, I.T. - Meldau, S.
Cytokinin levels and signaling respond to wounding and the perception of herbivore elicitors in Nicotiana attenuata.
Journal of Integrative Plant Biology. Roč. 57, č. 2 (2015), s. 198-212. ISSN 1672-9072. E-ISSN 1744-7909
R&D Projects: GA ČR GA206/09/2062
Institutional support: RVO:61389030
Keywords : Arabidopsis thaliana * cytokinin * herbivore-associated molecular patterns
Subject RIV: ED - Physiology
Impact factor: 3.670, year: 2015

Nearly half a century ago insect herbivores were found to induce the formation of green islands by manipulating cytokinin (CK) levels. However, the response of the CK pathway to attack by chewing insect herbivores remains unclear. Here, we characterize the CK pathway of Nicotiana attenuata (Torr. ex S. Wats.) and its response to wounding and perception of herbivore-associated molecular patterns (HAMPs). We identified 44 genes involved in CK biosynthesis, inactivation, degradation, and signaling. Leaf wounding rapidly induced transcriptional changes in multiple genes throughout the pathway, as well as in the levels of CKs, including isopentenyladenosine and cis-zeatin riboside; perception of HAMPs present in the oral secretions (OS) of the specialist herbivore Manduca sexta amplified these responses. The jasmonate pathway, which triggers many herbivore-induced processes, was not required for these HAMP-triggered changes, but rather suppressed the CK responses. Interestingly CK pathway changes were observed also in systemic leaves in response to wounding and OS application indicating a role of CKs in mediating long distance systemic processes in response to herbivory. Since wounding and grasshopper OS elicited similar accumulations of CKs in Arabidopsis thaliana L., we propose that CKs are integral components of wounding and HAMP-triggered responses in many plant species.
Permanent Link: http://hdl.handle.net/11104/0248171