Whole-Cell Optical Biosensor for Mercury – Operational Conditions in Saline Water

0443171 - ÚCHP 2016 RIV PL eng J - Journal Article
Solovyev, Andrey - Kuncová, Gabriela - Demnerová, K.
Whole-Cell Optical Biosensor for Mercury – Operational Conditions in Saline Water.
Chemical Papers. Roč. 69, č. 1 (2015), s. 183-191. ISSN 0366-6352
R&D Projects: GA TA ČR TA03010544
Institutional support: RVO:67985858
Keywords : mercury detection assay * bioluminescent bioreporter * sea water
Subject RIV: CA - Inorganic Chemistry
Impact factor: 1.326, year: 2015

The present study demonstrates the influences of chlorides, fluorides and bromides of potassium and sodium on the growth and Hg2+-induced bioluminescence of bioreporter Escherichia coli ARL1. In a Luria-Bertani medium,cell growth was inhibited by concentrations of sodium and potassium fluorides above 0.2 mol L-1. The addition of NaCl increased cell tolerance to the toxic effects of fluorides and bromides. Lag periods of 10 h and more were observed for cultivations in LB without NaCl and with halides at concentrations lower than 0.06 mol L-1. In a phosphate buffer (PB), the bioluminescence of E. coli ARL1, induced with HgCl2, was increased by the addition of NaCl, KCl, NaBr, KBr, NaF and KF (concentration of 0-0.25 mol L-1). In a saline phosphate buffer (PBS), the maxima of induced bioluminescence declined to 50%, in the case of NaF (0.12 mol L-1), and to zero for KF. An addition of tryptone to the induction medium increased induced light emission ten-fold. Concentrated artificial sea water (ASW) (70-100% ASW) inhibited bioluminescence induction. The new detection assay with E. coli ARL1 made possible the detection of 0.57 mu g L-1 of HgCl2 in double-diluted artificial sea water (25 % ASW).
Permanent Link: http://hdl.handle.net/11104/0245934