Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L

0443166 - ÚVGZ 2016 RIV CH eng J - Journal Article
Soural, I. - Vrchotová, Naděžda - Tříska, Jan - Balík, J. - Horník, Štěpán - Cuřínová, Petra - Sýkora, Jan
Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L.
Molecules. Roč. 20, č. 4 (2015), s. 6093-6112. E-ISSN 1420-3049
R&D Projects: GA MŠMT(CZ) LD14038
Institutional support: RVO:67179843 ; RVO:67985858
Keywords : Vitis vinifera L * grape cane * stilbenes * accelerated solvent extraction (ASE) * microwave-assisted extraction (MAE) * LC-MS
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.465, year: 2015

Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol.
Permanent Link: http://hdl.handle.net/11104/0245911