Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.)

0441012 - ÚEB 2015 RIV US eng J - Journal Article
Cápal, Petr - Ondřej, V.
Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.).
In Vitro Celluar and Developmental Biology - Plant. Roč. 50, č. 6 (2014), s. 789-794. ISSN 1054-5476. E-ISSN 1475-2689
Institutional support: RVO:61389030
Keywords : SOMATIC EMBRYOGENESIS * DNA METHYLATION * CHROMATIN
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.981, year: 2014

Cellular dedifferentiation is a process in which cells change from a given differentiated state into a transient, stem cell-like state and reenter into the cell cycle. In plants, cellular dedifferentiation is exemplified by protoplastization. Here, the cell wall of differentiated leaf cells is degraded by given enzymes that finish by protoplasts’ formation. The protoplasts assume a unique transient state with features resembling animal stem cells. The global chromatin decondensation is characterized for plant protoplasts that is also a distinguishing feature of animal stem cells. Various stimuli, that include phytohormones like auxins and cytokinins, induce protoplasts to reenter the cell cycle and proliferate. The reprogrammed cells could form calli, which under appropriate conditions can form shoots and roots, somatic embryos, and eventually fertile plant. However, application of auxins induces endoreduplication that could thwart future cell development; absence of these growth factors in culture of dedifferentiating protoplast cells causes their death with hallmark events of programmed cell death.
Permanent Link: http://hdl.handle.net/11104/0244074