Comparison of Fractionation Techniques of CO2 Extracts from Eucalyptus Globulus - Composition and Insecticidal Activity

0440668 - ÚCHP 2015 RIV NL eng J - Journal Article
Topiař, Martin - Sajfrtová, Marie - Pavela, R. - Machalová, Zdeňka
Comparison of Fractionation Techniques of CO2 Extracts from Eucalyptus Globulus - Composition and Insecticidal Activity.
Journal of Supercritical Fluids. Roč. 97, FEB 2015 (2015), s. 202-210. ISSN 0896-8446. E-ISSN 1872-8162
R&D Projects: GA TA ČR TA01010578
Institutional support: RVO:67985858
Keywords : supercritical fractinacion * eucalyptus globulus * insecticidal activity
Subject RIV: CI - Industrial Chemistry, Chemical Engineering
Impact factor: 2.579, year: 2015

Supercritical fluid extraction combined with two fractionation methods, sorption and series of separators, was applied on Eucalyptus globulus leaves in order to maximize the insecticidal activity of extracts against Leptinotarasa decemlineata larvae. The major compounds in the essential oil isolated with the yield 26.7 mg/g were 1,8-cineole (42.6 wt%), -pinene (10.7 wt%) and globulol (5.9 wt%). The concentration of total volatiles in the extracts varied from 2.8 to 59.5 wt%, depending on the extraction and fractionation conditions. The extract obtained at 50 °C and 9 MPa exhibited the highest acute toxicity with LD50 = 38 µg. ScCO2 extracts exhibited several times stronger insecticidal activity in terms of chronic toxicity, antifeedant activity and growth inhibition than essential oil. Increase in the concentration of volatiles in isolates resulted into increase of chronic toxicity and antifeedant activity while growth inhibition decreased.
Permanent Link: http://hdl.handle.net/11104/0244835