Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

0440405 - BFÚ 2015 RIV AT eng J - Journal Article
Nevrtalová, Eva - Baloun, Jiří - Hudzieczek, Vojtěch - Čegan, Radim - Vyskot, Boris - Doležel, Jaroslav - Šafář, Jan - Milde, D. - Hobza, Roman
Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes.
Protoplasma. Roč. 251, č. 6 (2014), s. 1427-1439. ISSN 0033-183X. E-ISSN 1615-6102
R&D Projects: GA ČR(CZ) GAP501/12/2220; GA ČR(CZ) GBP501/12/G090; GA ČR(CZ) GP13-34962P; GA ČR(CZ) GA522/09/0083
Institutional support: RVO:68081707
Keywords : Copper * Gene duplication * Metallothionein
Subject RIV: BO - Biophysics; EF - Botanics (UEB-Q)
Impact factor: 2.651, year: 2014

Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress.
Permanent Link: http://hdl.handle.net/11104/0243516