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Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)

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    0433555 - ÚEB 2015 RIV US eng J - Journal Article
    Ouyang, S.H. - Zhang, D. - Han, J. - Zhao, X.J. - Cui, Y. - Song, W. - Keeble-Gagnere, G. - Appels, R. - Doležel, Jaroslav - Ling, H.Q. - Sun, Q.X. - Liu, Z.Y. … Total 25 authors
    Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides).
    PLoS ONE. Roč. 9, č. 6 (2014). ISSN 1932-6203. E-ISSN 1932-6203
    Institutional support: RVO:61389030
    Keywords : TURGIDUM VAR. DICOCCOIDES * CHROMOSOME BIN MAP * SEQUENCE TAG LOCI
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 3.234, year: 2014

    Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172.
    Permanent Link: http://hdl.handle.net/11104/0237740

     
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