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UHPLC-MS/MS based target profiling of stress-induced phytohormones

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    0433242 - ÚEB 2015 RIV GB eng J - Journal Article
    Floková, Kristýna - Tarkowská, Danuše - Miersch, Otto - Strnad, Miroslav - Wasternack, Claus - Novák, Ondřej
    UHPLC-MS/MS based target profiling of stress-induced phytohormones.
    Phytochemistry. Roč. 105, SEP 2014 (2014), s. 147-157. ISSN 0031-9422
    R&D Projects: GA ČR GA14-34792S; GA MŠk(CZ) LO1204; GA MŠk LK21306
    Institutional support: RVO:61389030
    Keywords : Stress-induced phytohormones * Jasmonates * Abscisic acid
    Subject RIV: CB - Analytical Chemistry, Separation
    Impact factor: 2.547, year: 2014

    Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight. FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA.
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