Endogenous cytokinin profiles of tissue-cultured and acclimatized 'Williams' bananas subjected to different aromatic cytokinin treatments

0431516 - ÚEB 2015 RIV IE eng J - Journal Article
Aremu, A.O. - Plačková, Lenka - Bairu, M. W. - Novák, Ondřej - Szüčová, Lucie - Doležal, Karel - Finnie, J. F. - van Staden, J.
Endogenous cytokinin profiles of tissue-cultured and acclimatized 'Williams' bananas subjected to different aromatic cytokinin treatments.
Plant Science. Roč. 214, JAN 2014 (2014), s. 88-98. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA MŠMT(CZ) LO1204
Grant - others:GA MŠk(CZ) ED0007/01/01
Program: ED
Institutional support: RVO:61389030
Keywords : Micropropagation * Musa spp. * Phytohormones
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.607, year: 2014

Endogenous cytokinin (CK) levels of in vitro-cultured and greenhouse-acclimatized 'Williams' bananas treated with six aromatic CKs were quantified using UPLC-MS/MS. The underground parts had higher endogenous CK levels than the aerial parts. Control plantlets had more isoprenoid CKs while the aromatic-type CKs were predominant in all other regenerants. Following acclimatization of the control and 10 mu M CK regenerants, there was a rapid decline in both isoprenoid and aromatic CK in the greenhouse-grown plants. Apart from the control and 6-(3-Methoxybenzylamino)-9-tetrahydropyran-2-ylpurine (MemTTHP) treatment with higher level of isoprenoid CK, aromatic CK remain the predominant CK-type across all CK treatments. The most abundant CK forms were meta-topolin (mT) and benzyladenine (BA) in the micropropagated and acclimatized plants, respectively. Micropropagated plantlets had cis-Zeatin (cZ) as the major isoprenoid CK-type which was in turn replaced by isopentenyladenine (iP) upon acclimatization. On a structural and functional basis, 9-glucoside, a deactivation/detoxicification product was the most abundant and mainly located in the underground parts (micropropagation and acclimatization). The results establish the wide variation in metabolic products of the tested aromatic CKs during micropropagation and acclimatization. The findings are discussed with the possible physiological roles of the various CK constituents on the growth and development of banana plants.
Permanent Link: http://hdl.handle.net/11104/0236118