The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells

0429989 - ÚEB 2015 RIV CH eng J - Journal Article
Synek, Lukáš - Sekereš, Juraj - Žárský, Viktor
The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells.
Frontiers in Plant Science. Roč. 4, JAN 2014 (2014). ISSN 1664-462X. E-ISSN 1664-462X
R&D Projects: GA ČR GPP501/11/P853
Institutional support: RVO:61389030
Keywords : exocyst * actin cytoskeleton * microtubule cytoskeleton
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.948, year: 2014

Delivery and final fusion of the secretory vesicles with the relevant target membrane are hierarchically organized and reciprocally interconnected multi-step processes involving not only specific protein protein interactions, but also specific protein phospholipid interactions. The exocyst was discovered as a tethering complex mediating initial encounter of arriving exocytic vesicles with the plasma membrane. The exocyst complex is regulated by Rab and Rho small GTPases, resulting in docking of exocytic vesicles to the plasma membrane (PM) and finally their fusion mediated by specific SNARE complexes. In model Opisthokont cells, the exocyst was shown to directly interact with both microtubule and microfilament cytoskeleton and related motor proteins as well as with the PM via phosphatidylinositol 4,5-bisphosphate specific binding, which directly affects cortical cytoskeleton and PM dynamics. Here we summarize the current knowledge on exocyst-cytoskeleton-PM interactions in order to open a perspective for future research in this area in plant cells.
Permanent Link: http://hdl.handle.net/11104/0234933