Impact of Wines and Wine Constituents on Cyclooxygenase-1, Cyclooxygenase-2, and 5-Lipoxygenase Catalytic Activity

0429537 - ÚEB 2015 RIV GB eng J - Journal Article
Kutil, Zsófia - Temml, V. - Maghradze, D. - Přibylová, Marie - Dvořáková, Marcela - Schuster, D. - Vaněk, Tomáš - Landa, Přemysl
Impact of Wines and Wine Constituents on Cyclooxygenase-1, Cyclooxygenase-2, and 5-Lipoxygenase Catalytic Activity.
Mediators of Inflammation. Roč. 2014, UNSP 178931 (2014). ISSN 0962-9351. E-ISSN 1466-1861
R&D Projects: GA MŠMT(CZ) LD11005; GA MŠMT 7AMB13AT008
Institutional support: RVO:61389030
Keywords : HEART-DISEASE * PLATELET-AGGREGATION * CARDIOVASCULAR-DISEASE
Subject RIV: GM - Food Processing
Impact factor: 3.236, year: 2014

Cyclooxygenases and lipoxygenases are proinflammatory enzymes; the former affects platelet aggregation, vasoconstriction, vasodilatation and later the development of atherosclerosis. Red wines from Georgia and central and western Europe inhibited cyclooxygenase-1 (COX-1) activity in the range of 63-94%, cyclooxygenase-2 (COX-2) activity in the range of 20-44% (tested at a concentration of 5 mL/L), and 5-lipoxygenase (5-LOX) activity in the range of 72-84% (at a concentration of 18.87 mL/L). White wines inhibited 5-LOX in the range of 41-68% at a concentration of 18.87 mL/L and did not inhibit COX-1 and COX-2. Piceatannol (IC50 = 0.76 mu M) was identified as a strong inhibitor of 5-LOX followed by luteolin (IC50 = 2.25 mu M), quercetin (IC50 = 3.29 mu M), and myricetin (IC50 - 4.02 mu M). trans-Resveratrol was identified as an inhibitor of COX-1 (IC50 - 2.27 mu M) and COX-2 (IC50 - 3.40 mu M). Red wine as a complex mixture is a powerful inhibitor of COX-1, COX-2, and 5-LOX, the enzymes involved in eicosanoid biosynthetic pathway.
Permanent Link: http://hdl.handle.net/11104/0234629