LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

0424009 - ÚCHP 2014 RIV US eng J - Journal Article
Horník, Štěpán - Sajfrtová, Marie - Karban, Jindřich - Sýkora, Jan - Březinová, Anna - Wimmer, Zdeněk
LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts.
Journal of Analytical Methods in Chemistry. Roč. 2013, č. 2013 (2013), ID 526818. ISSN 2090-8865. E-ISSN 2090-8873
R&D Projects: GA TA ČR TA01010578
Grant - others:GA ČR(CZ) GAP503/11/0616
Program: GA
Institutional support: RVO:67985858 ; RVO:61388963 ; RVO:61389030
Keywords : LC-NMR technique * phytosterols * analyses
Subject RIV: CC - Organic Chemistry; EE - Microbiology, Virology (UEB-Q)
Impact factor: 0.948, year: 2013

The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterolswere isolated and analyzed by isocraticHPLC-NMRmethods.Theresults of qualitative and quantitative analyses were in a good agreement with the literature data.
Permanent Link: http://hdl.handle.net/11104/0230085