Endogenous progesterone and its cellular binding sites in wheat exposed to drought stress

0423122 - ÚEB 2014 RIV GB eng J - Journal Article
Janeczko, A. - Oklešťková, Jana - Siwek, A. - Dziurka, M. - Pociecha, E. - Kocurek, M. - Novák, Ondřej
Endogenous progesterone and its cellular binding sites in wheat exposed to drought stress.
Journal of Steroid Biochemistry and Molecular Biology. Roč. 138, č. 2013 (2013), s. 384-394. ISSN 0960-0760
R&D Projects: GA MŠMT LK21306
Institutional research plan: CEZ:AV0Z50380511
Keywords : Plants * Wheat * Progesterone
Subject RIV: EF - Botanics
Impact factor: 4.049, year: 2013

Progesterone is a basic hormone that regulates the metabolism in mammals. The presence of this compound has also been found in certain plants. It is believed that progesterone can regulate growth processes and resistance to stress, however, its precise role in plants remains unknown. The research conducted in this study was aimed at analyzing the content of endogenous progesterone and its cellular binding sites in the leaves of spring wheat exposed to drought. Changes were studied in two cultivars of wheat - a cultivar sensitive to drought (Katoda) and tolerant cultivar (Monsun). Plants had undergone periodic droughts during the seedling stage or in the phase of heading. The occurrence of free progesterone as well as its conjugated forms was observed in wheat studied. The amount of progesterone ranged from 0.2 to 5.8 pmol g FW-1 and was dependent on the cultivar, age of the plants, stage of development and fluctuated as a result of the exposure to drought. Cv. Katoda responded to a water deficit by lowering the amount of progesterone and cv. Monsun by increasing its level. Progesterone in plants grown in limited water conditions occurred primarily in a free form. While in the optimal watering conditions, some of its pool was found in the form of conjugates. In the spring wheat the occurrence of binding sites for progesterone was detected in cell membranes, cytoplasm and nuclei in the range of 10-36 fmol/mg of protein. The wheat cultivars tested, Monsun and Katoda, differ in their concentration of cellular binding sites for progesterone. This number varied in the individual fractions during different stages of plant development and due to the effect of drought stress. The number of binding sites for progesterone located in the membrane fraction of seedlings and flag leaves increased significantly under drought in the cv. Katoda (35-46%), but did not change in the cv. Monsun.
Permanent Link: http://hdl.handle.net/11104/0229357