A Physical Map of the Short Arm of Wheat Chromosome 1A

0423119 - ÚEB 2014 RIV US eng J - Journal Article
Breen, J. - Wicker, T. - Shatalina, M. - Frenkel, Z. - Bertin, I. - Philippe, R. - Spielmeyer, W. - Šimková, Hana - Šafář, Jan - Cattonaro, F. - Scalabrin, S. - Magni, F. - Vautrin, S. - Berges, H. - Paux, E. - Fahima, T. - Doležel, Jaroslav - Korol, A. - Feuillet, C. - Keller, B.
A Physical Map of the Short Arm of Wheat Chromosome 1A.
PLoS ONE. Roč. 8, č. 11 (2013). ISSN 1932-6203. E-ISSN 1932-6203
Institutional research plan: CEZ:AV0Z50380511
Keywords : TRITICUM-AESTIVUM L. * EXPRESSED SEQUENCE TAGS * WEIGHT GLUTENIN LOCI
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.534, year: 2013

Bread wheat (Triticum aestivum) has a large and highly repetitive genome which poses major technical challenges for its study. To aid map-based cloning and future genome sequencing projects, we constructed a BAC-based physical map of the short arm of wheat chromosome 1A (1AS). From the assembly of 25,918 high information content (HICF) fingerprints from a 1AS-specific BAC library, 715 physical contigs were produced that cover almost 99% of the estimated size of the chromosome arm. The 3,414 BAC clones constituting the minimum tiling path were end-sequenced. Using a gene microarray containing similar to 40 K NCBI UniGene EST clusters, PCR marker screening and BAC end sequences, we arranged 160 physical contigs (97 Mb or 35.3% of the chromosome arm) in a virtual order based on synteny with Brachypodium, rice and sorghum. BAC end sequences and information from microarray hybridisation was used to anchor 3.8 Mbp of Illumina sequences from flow-sorted chromosome 1AS to BAC contigs. Comparison of genetic and synteny-based physical maps indicated that similar to 50% of all genetic recombination is confined to 14% of the physical length of the chromosome arm in the distal region. The 1AS physical map provides a framework for future genetic mapping projects as well as the basis for complete sequencing of chromosome arm 1AS.
Permanent Link: http://hdl.handle.net/11104/0229355