Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation >

0422255 - BFÚ 2014 RIV GB eng J - Journal Article
Černý, M. - Kuklová, A. - Hoehenwarter, W. - Fragner, L. - Novák, Ondřej - Rotková, Gabriela - Jedelský, P. - Žáková, K. - Šmehilová, M. - Strnad, Miroslav - Weckwerth, W. - Brzobohatý, Břetislav
Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation >.
Journal of Experimental Botany. Roč. 64, č. 14 (2013), s. 4193-4206. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA ČR(CZ) GA206/09/2062; GA MŠMT(CZ) ED1.1.00/02.0068
Grant - others:GA ČR(CZ) GAP305/12/2144
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : INDUCIBLE GENE-EXPRESSION * BETA-GLUCOSIDASE * PLANT-GROWTH
Subject RIV: BO - Biophysics; EF - Botanics (UEB-Q)
Impact factor: 5.794, year: 2013

In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S > GR > HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S > GR > ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified > 1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development, and/or hormone and light signalling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates, and organic acids.
Permanent Link: http://hdl.handle.net/11104/0228496