Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits

0421944 - ÚEB 2014 RIV US eng J - Journal Article
Kosová, K. - Vítámvás, P. - Planchon, S. - Renaut, J. - Vaňková, Radomíra - Prášil, I.T.
Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits.
Journal of Proteome Research. Roč. 12, č. 11 (2013), s. 4830-4845. ISSN 1535-3893. E-ISSN 1535-3907
R&D Projects: GA ČR GA522/09/2058
Institutional research plan: CEZ:AV0Z50380511
Keywords : 2D-DIGE analysis * cold stress * spring and winter growth habit
Subject RIV: ED - Physiology
Impact factor: 5.001, year: 2013

A proteomic response to cold treatment (4 degrees C) has been studied in crowns of a frost-tolerant winter wheat cultivar Samanta and a frost-sensitive spring wheat cultivar Sandra after short-term (3 days) and long-term (21 days) cold treatments. Densitometric analysis of 2-D differential in gel electrophoresis (2D-DIGE) gels has resulted in the detection of 386 differentially abundant protein spots, which reveal at least a two-fold change between experimental variants. Of these, 58 representative protein spots have been selected for MALDI-TOF/TOF identification, and 36 proteins have been identified. The identified proteins with an increased relative abundance upon cold in both growth habits include proteins involved in carbohydrate catabolism (glycolysis enzymes), redox metabolism (thioredoxin-dependent peroxidase), chaperones, as well as defense-related proteins (protein revealing similarity to thaumatin). Proteins exhibiting a cold-induced increase in the winter cultivar include proteins involved in regulation of stress response and development (germin E, lectin VER2), while proteins showing a cold-induced increase in the spring cultivar include proteins involved in restoration of cell division and plant growth (eIF5A2, glycine-rich RNA-binding protein, adenine phosphoribosyltransferase). These results provide new insights into cold acclimation in spring and winter wheat at the proteome level and enrich our previous work aimed at phytohormone dynamics in the same plant material.
Permanent Link: http://hdl.handle.net/11104/0228190