Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn

0421942 - ÚEB 2014 RIV NL eng J - Journal Article
Trifunovic, M. - Cingel, A. - Simonovic, A. - Jevremovic, S. - Petric, M. - Dragicevic, I. - Motyka, Václav - Dobrev, Petre - Zahajská, Lenka - Subotic, A.
Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.
Plant Cell, Tissue and Organ Culture. Roč. 115, č. 2 (2013), s. 139-150. ISSN 0167-6857. E-ISSN 1573-5044
R&D Projects: GA ČR(CZ) GAP506/11/0774
Institutional research plan: CEZ:AV0Z50380511
Keywords : Centaurium erythraea Rafn. * Agrobacterium tumefaciens * Genetic transformation
Subject RIV: EF - Botanics
Impact factor: 2.612, year: 2013

Cytokinin oxidase/dehydrogenase (CKX) is the only known enzyme involved in cytokinin catabolism. Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were introduced separately into a binary cloning vector, immobilized into Agrobacterium tumefaciens strain GV3101, and introduced into root explants of centaury (Centaurium erythraea Rafn.). The integration of each transgene was confirmed by genomic PCR. Of the total transformed explants, 30 and 28.2 % of the transformants carried AtCKX1 and AtCKX2 transgenes, respectively. Of these transformants, 50 % exhibited expression of the AtCKX1 transgene, while 64 % of transformants exhibited expression of the AtCKX2 transgene. For all analysed AtCKX transgenic centaury lines, as well as for untransformed control plants, CKX activity was higher in roots than in shoots. Expression of AtCKX in most transgenic lines contributed to enhanced levels of CKX activity in root tissues; whereas, only a few lines demonstrated increased CKX activity in shoot tissues compared to those of control plants. Moreover, overexpression of AtCKX resulted in reduced morphogenetic potential in transgenic plants, but did not significantly affect biomass production in comparison to untransformed control plants.
Permanent Link: http://hdl.handle.net/11104/0228210